pmemerald nup50 construct Search Results


pmemerald nup50 construct  (Addgene inc)
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Addgene inc pmemerald nup50 construct
Confocal images of living HeLa cells expressing PENELOPE fusion proteins. Cells were incubated with 25 µM BV for 2 h prior to imaging ( A ) Cytokeratin-18, ( B ) Vimentin, ( C ) Microtubules (α-Tubulin), ( D ) Microtubule ends (EB3), ( E ) Zyxin, ( F ) Actin (LifeAct), ( G ) Mitochondria (Omp25), ( H ) Mitochondria (Cox8a), ( I ) Nuclear pores <t>(Nup50)</t> ( J ) Histones (Histone H2B type 1-N), ( K ) Lysosomes (Lamp1) and ( L ) Peroxisomes. Background subtraction was performed on ( D , E , I , K , and L ). (Scale bar: 10 μm.)
Pmemerald Nup50 Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmemerald+nup50+construct/mEmerald-Nup50-C-10+(Plasmid+%2354209)/pmc12685048-187-18-29
Average 92 stars, based on 1 article reviews
pmemerald nup50 construct - by Bioz Stars, 2026-10
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Confocal images of living HeLa cells expressing PENELOPE fusion proteins. Cells were incubated with 25 µM BV for 2 h prior to imaging ( A ) Cytokeratin-18, ( B ) Vimentin, ( C ) Microtubules (α-Tubulin), ( D ) Microtubule ends (EB3), ( E ) Zyxin, ( F ) Actin (LifeAct), ( G ) Mitochondria (Omp25), ( H ) Mitochondria (Cox8a), ( I ) Nuclear pores (Nup50) ( J ) Histones (Histone H2B type 1-N), ( K ) Lysosomes (Lamp1) and ( L ) Peroxisomes. Background subtraction was performed on ( D , E , I , K , and L ). (Scale bar: 10 μm.)

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: The near-infrared bacteriophytochrome-derived fluorescent protein PENELOPE enables RESOLFT superresolution microscopy

doi: 10.1073/pnas.2504748122

Figure Lengend Snippet: Confocal images of living HeLa cells expressing PENELOPE fusion proteins. Cells were incubated with 25 µM BV for 2 h prior to imaging ( A ) Cytokeratin-18, ( B ) Vimentin, ( C ) Microtubules (α-Tubulin), ( D ) Microtubule ends (EB3), ( E ) Zyxin, ( F ) Actin (LifeAct), ( G ) Mitochondria (Omp25), ( H ) Mitochondria (Cox8a), ( I ) Nuclear pores (Nup50) ( J ) Histones (Histone H2B type 1-N), ( K ) Lysosomes (Lamp1) and ( L ) Peroxisomes. Background subtraction was performed on ( D , E , I , K , and L ). (Scale bar: 10 μm.)

Article Snippet: The Nup50 fusion construct was cloned by exchanging mEmerald with the PCR product via NheI and BglII in pmEmerald –Nup50 construct (this plasmid was a gift from Michael Davidson, Addgene plasmid # 54209).

Techniques: Expressing, Incubation, Imaging